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In Vivo Monitoring of Fluorinated Inhalational Anesthetics Using 19F MRI: A Pilot Study

B Bednarz

B Bednarz*, K Ludwig , S Fain , Department of Medical Physics, University of Wisconsin, Madison, WI


SU-E-QI-9 Sunday 3:00PM - 6:00PM Room: Exhibit Hall

Purpose: Despite the widespread use of fluorinated hydrocarbons for inhalational anesthesia very little is known about the role of these anesthetics in postoperative cognitive impairment. There is a compelling need for safe and effective quantitative and qualitative imaging techniques to monitor the spatial distribution of inhalational anesthetics in vivo to appropriately assess this role. Herein we present a pilot study on the use of ¹⁹F MRI to image fluorinated anesthetics in vivo.

Methods: The spatial distribution of isoflurane in a healthy C57BL/6 mouse subject to a constant flow rate of gas was image using a 4.7T small animal MRI system (Aligent Technologies, Santa Clara, CA). Multiple coronal 19F 2 mm slice images were acquired using a volumetric quadrature coil tunable to ¹⁹F frequency (187.9 MHz) averaging over 4 different time points (30, 35, 60 and 75 minutes). ¹⁹F images were overlaid onto T2-weighted fast echo multi-slice (FSEMS) ¹H images of soft tissue anatomy.

Results: An average over the 4 collection time points clearly demonstrates the distribution of isoflurane in vivo with peak uptake in muscle and fatty tissue as well as in the frontal lobe of the mouse brain. A time resolved imaging sequence demonstrates the increasing uptake of isoflurane in each of these compartments.

Conclusion: A novel technique to image fluorinated gas anesthetics in vivo has been demonstrated. To the best of our knowledge, this is the first time isoflurane has been imaged in vivo using MRI.

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