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Quantification of Nanoparticle Heating and Concentration for MR-Guided Laser Interstitial Thermal Therapy

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C MacLellan

CJ MacLellan1,2*, M Melancon1,2 , F Salatan1 , Q Yang1 , KP Hwang3 , D Fuentes1,2 , RJ Stafford1,2 , (1) The University of Texas MD Anderson Cancer Center, Houston, TX, (2) The University of Texas Graduate School of Biomedical Sciences at Houston, (3) GE Healthcare, Waukesha, Wisconsin


MO-FG-BRA-9 (Monday, July 13, 2015) 4:30 PM - 6:00 PM Room: Ballroom A

Purpose: Nanoparticle Mediated Laser Interstitial Thermal Therapy (npLITT) is a technique that utilizes tumor localized optically activated nanoparticles to increase the conformality of laser ablation procedures. Temperatures in these procedures are dependent on the particle concentration which generally cannot be measured noninvasively prior to therapy. In this work we attempt to quantify particle concentration in vivo by estimating the increase in R2* relaxation induced by bifunctional magnetic resonance (MR)-visible gold-based nanoparticles (SPIO@Au) and relate it to the temperature increase observed during real time MR temperature imaging (MRTI) of laser ablation.

Methods: SPIO@Au nanoparticles (90nm) were synthesized containing a silica-iron core (for MR visibility via R2*) and gold shell (for near-infrared absorption). High resolution R2* maps were acquired before and after injecting four different particle concentrations (saline,1e10, 5e10, and 10e10 particles/mL) into HN5 flank xenografts. Tumors were monitored using MRTI during treatment with an interstitial fiber. (1 watt, 808 nm, 3 minutes)

Results: The maximum temperature within the tumors increased linearly with concentration of injected particles, reaching 34.0, 37.6, 45.8, and 55.4 ⁰C for saline, 1e10, 5e10 and 10e10 particles/mL injections, respectively (R2=.994). The highest temperatures occur at the injection site rather than the fiber, confirming that SPIO@Au nanoparticles are the primary absorber. The differences between the median R2* measured at the injection site and the rest of the tumor were -6, 134, 111, 156 s-1 for the saline,1e10,5e10 and 10e10 particles/mL injections, respectively. This R2* change is consistent with the measured relaxivity for the 1e10 particles/mL injection but does not maintain linearity at higher concentrations.

Conclusion: Bifunctional SPIO@Au nanoparticles are a promising technology for providing noninvasive estimates of particle concentration via MRI and temperature increase in npLITT procedures. Future experiments will focus on lower, physiologically relevant particle concentrations and spin echo R2 mapping to better quantify the particle concentration.

Funding Support, Disclosures, and Conflict of Interest: This research was supported by the National Institutes of Health and National Cancer Institute under Award Numbers TL1TR000369 and P30CA016672 and was conducted at the MD Anderson Center for Advanced Biomedical Imaging in-part with equipment support from General Electric Healthcare.

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