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In Vivo Bioluminescence Tomography for Small Animal Radiation Research Platform (SARRP)

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Z Deng

Z Deng1*, X Xu1 , T Garzon-Muvdi1 , A Luksik1 , R Maxwell1 , I Iordachita2 , M Lim1 , J Wong1 , K Wang1 , (1) Johns Hopkins School of Medicine, Baltimore, Maryland, (2) Johns Hopkins University, Baltimore, Maryland


TU-C1-GePD-IT-3 (Tuesday, August 1, 2017) 9:30 AM - 10:00 AM Room: Imaging ePoster Theater

Purpose: Although cone beam CT(CBCT) provides strong contrast using bony landmark, it is still challenging to localize soft tissue targets. To address this shortcoming, we developed a 3D bioluminescence tomography(BLT) to guide SARRP radiation for soft tissue targets. In this study, we used a glioblastoma multiforme(GBM) mouse model as the in vivo platform to assess the localization/targeting accuracy of the BLT-SARRP system.

Methods: Our BLT system consists of an optical assembly, a mobile cart and a moveable mouse bed. The 3-mirror design in the optical assembly can rotate 180° around animal and capture multiple projections images. CBCT images were acquired to provide anatomic profile of the animal as well as to generate numerical mesh for BLT reconstruction. To establish the GBM model, GL261-Luc cells were stereotactically injected in a 1-μL volume into the left striatum. Mice were imaged 3 weeks after cells implantation. Multispectral images were acquired to improve BLT reconstruction accuracy. To find the optimal imaging workflow and reconstruction accuracy, we compared the BLT localization accuracy between single projection images acquired at four wavelengths vs. multiple projections images acquired at two wavelengths. After bioluminescence imaging, mice were transferred to SARRP for CBCT acquisition and BLT-guided radiation delivery. The BLT reconstructed center of mass (CoM) of the GBM was compared with (1) the origin of the surgically implanted region; (2) CoM calculated from CT contrast image; (3) the center of the γH2AX DNA strand break staining in response to BLT-guided radiation delivery.

Results: Our preliminary results show that with single projection multispectral images, the BLT reconstructed CoM of the GBM model is at accuracy of 1.1±0.2mm (n=6), compared with the center of the surgically implanted region.

Conclusion: This study will demonstrate that the BLT-SARRP system could be an innovative pre-clinical system to localize soft tissue targets and guide focal irradiation.

Funding Support, Disclosures, and Conflict of Interest: This work was supported by Xstrahl Ltd (90043185). Drs. Wong and Iordachita receive royalty payment from a licensing agreement between Xstrahl Ltd. and Johns Hopkins University.

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