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Fluorescence Tomography-Small Animal Radiation Research Platform (SARRP) for Pre-Clinical Radiation Research

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Z Deng

Z Deng1*, I Iordachita2 , X Xu1 , J Wong1 , K Wang1 , (1) Johns Hopkins School of Medicine, Baltimore, Maryland, (2) Johns Hopkins University, Baltimore, Maryland


SU-K-702-12 (Sunday, July 30, 2017) 4:00 PM - 6:00 PM Room: 702

Purpose: The small animal radiation research platform (SARRP) developed by our group has advanced pre-clinical radiation research by mimicking human treatment. With the increasing pool of reporters, fluorescence imaging allows important mechanistic study of various response pathways in vivo. To improve the functional imaging and target localization capability of the SARRP, it is compelling to incorporate 3D fluorescence tomography (FT) to the SARRP such that the response of a functional target can be assessed in accurate registration to the irradiated volume. In this work, we described the design of an off-line FT system and system validation.

Methods: Our FT system consists of a laser-galvo system, a vertical mouse bed, an optical acquisition assembly and a mobile cart (Fig.1). The galvo system can direct a collimated laser and scan the whole mouse at large scanning field (11cm×11cm). The laser excites the fluorescent reporter and induces the fluorescence captured by our filter-lens-camera assembly. The mouse bed is motorized to rotate around vertical axis for multiple projections images. After optical imaging, animal with the bed will be transferred to the SARRP for cone beam CT (CBCT) imaging. The CBCT is acquired for SARRP dose calculation as well as to generate numerical mesh for FT reconstruction. To validate the FT system, we first use a mouse-shaped phantom with 2 fluorescent sources placed inside. The FT reconstructed 3D source positions will be compared with that of CBCT image. We will further validate the FT system using animal model.

Results: The FT design is shown in Figure 1. We are also testing the stability of the mouse position during the mouse bed transferred between FT and SARRP.

Conclusion: This study will demonstrate the feasibility of using the off-line FT system for SARRP radiation guidance and for longitudinally study without interfering SARRP to optimize experiment throughput.

Funding Support, Disclosures, and Conflict of Interest: This work was supported by Xstrahl Ltd (90043185). Drs. Wong and Iordachita receive royalty payment from a licensing agreement between Xstrahl Ltd. and Johns Hopkins University.

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