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Gold Nanoparticle Radio-Sensitization of MCF-7 Breast Cancer Cells

N Wen

B Janic1 , S Brown1 , H Bagher-Ebadian1 , F Liu2 , G Mao2 , I Chetty1 , B Movsas1 , N Wen1*, (1) Henry Ford Health System, Detroit, MI, (2) Wayne State University, Detroit, MI


MO-DE-605-4 (Monday, July 31, 2017) 1:45 PM - 3:45 PM Room: 605

Purpose: Nanoparticles are structures 1-100 nm in size explored for their application in cancer diagnosis and treatment. Our long-term plan is to develop nanoparticles that will simultaneously serve as imaging and radio-sensitization probes. In the current study, we explore the ability of gold nanoparticles (AuNP), of two different sizes, to increase the sensitivity of breast cancer cells to three different megavoltage (MV) X-rays.

Methods: The effect of 4 and 14nm diameter AuNPs on MCF-7 cell’s viability and response to radiation was studied. Cells were incubated for 6 and 24 hours with 4 and 14nm AuNP at two different concentrations. Cells were irradiated using 4Gy X-rays of 2.5flattening filter free (FFF), 6 or 10FFF MV energies. Cells were then allowed to grow for 3 or 7 days after which cell proliferation was analyzed by MTT assay. For viability studies, the proliferation of non-irradiated cells exposed to AuNP alone was assessed.

Results: AuNP alone was not toxic to MCF-7 cells. Radio-sensitization, in contrast, was significant and most evident 7 days after irradiation. In cells incubated for 6h with 4nm AuNP, a significant dose enhancement of 27% and 31% was observed at 6 and 10 MV, respectively. Under the same conditions, 14nm AuNP induced a significant enhancement of 49% at 6MV and 37% at 10 MV. In cells incubated for 24h with 4nm AuNP, a significant dose enhancement of 18% was detected using 2.5 and 10 MV X-rays, while 14nm AuNP induced 52% significant dose enhancement at 2.5 MV only.

Conclusion: AuNP radiosenzitization of MCF-7 cells was most pronounced 7 days after irradiation at all energies investigated, especially after 2.5MV X-rays. The magnitude of radiosensitization was dependent on AuNP size, concentration and incubation time. The lack of significant cytotoxicity in the absence of radiation provides a platform for further exploring AuNP radio-sensitizing potential.

Funding Support, Disclosures, and Conflict of Interest: The study was supported by a Research Scholar Grant, RSG-15-137-01-CCE from the American Cancer Society.

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